Furthermore, we have emphasized the application of CRISPR technology in virus diagnosis and in finding significant genes involved in virus-host interactions.Ĭlustered regularly interspaced short palindromic repeats (CRISPR)-Cas13 has drawn broad interest to control gene expression and cell fate at the RNA level in general. In this review, we have focused on the best and the most effective ways to design sgRNA, gene knock-in(s), and gene knock-out(s) for virus-targeted manipulation. Nevertheless, a valid and scientifically designed CRISPR system is critical to make more effective and accurate changes in viruses. Furthermore, this method can be used to make an effective and precise diagnosis of viral infections. This approach has provided an unprecedented opportunity for creating simple, inexpensive, specific, targeted, accurate, and practical manipulations of viruses, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), human immunodeficiency virus-1 (HIV-1), and vaccinia virus.
CRISPR (clustered regularly interspaced short palindromic repeats) is becoming one of the most functional and widely used tools for RNA and DNA manipulation in multiple organisms.
However, researchers have been trying to manipulate viral genomes to overcome various disorders, including cancer, for vaccine development purposes. Viruses are one of the most important concerns for human health, and overcoming viral infections is a worldwide challenge.